Journal: International Journal of Molecular Sciences

Article Title: Acceleration of TAA-Induced Liver Fibrosis by Stress Exposure Is Associated with Upregulation of Nerve Growth Factor and Glycopattern Deviations

doi: 10.3390/ijms22105055

Figure Lengend Snippet: ( A ) For an analysis of the glycophenotype, lectins were used to detect different types of glycosylation. O-glycan structures were recognized by Helix pomatia agglutinin (HPA; Tn-antigen) and Arachis hypogaea lectin (PNA; core 1). Lycopersicon esculentum lectin (LEA) recognizes polyLAcNac sequences. Phaseolus vulgaris lectin (PHA-L) recognizes β1-6GlcNAc-branched complex N-glycans and Phaseolus vulgaris lectin E subunit (PHA-E) identifies glycans with a bisecting modification. Fucosylation was determined by Lotus tetragonolobus lectin (LTL) and finally sialyation was determined using the Maackia amurensis lectin (MAA) and Sambucus nigra agglutinin (SNA-I) which bind to α2,3- and α2,6-linked sialic acid, respectively. Quantification of O-glycan ( B ), fucosylation ( C ), N-glycosylation ( D ), or sialylated glycan ( E ) was performed on liver sections of mice with fibrosis (TAA), fibrosis and stress (TAA + stress), and fibrosis and NGF administration (TAA + NGF). Mean fluorescence intensity (MFI) ± S.E.M is shown. * p < 0.05 and *** p < 0.001 vs. TAA (Kruskal–Wallis test). Expression of MGAT5 ( F ) and C1GALT1 ( G ) in patients with fibrosis due to HCV (GSE6764), HBV (GSE84044), or NAFLD (GSE49541). * p < 0.05 and ** p < 0.01 (Mann–Whitney test).

Article Snippet: Quantitative analysis of collagen content was performed by computerized morphometric analysis on samples stained with Sirius red and digitally scanned by a high-resolution bright field and fluorescence slide scanner (Pannoramic MIDI BF/FL, 3DHISTECH Ltd., Budapest, Hungary).

Techniques: Glycoproteomics, Modification, Fluorescence, Expressing, MANN-WHITNEY